In order to determine whether the brain is undergoing neurogenesis, scientists needed to devise a way to mark proliferating cells.One way to do this is to identify cells that are in the S phase – also known as the “synthesis” phase – of replication.Brd U is a synthetic analogue of Thymidine that is also incorportated into cells during DNA replication, which allows us to successfully identify dividing cells.

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Birthdating cells when your parent starts dating

Together, these data suggest that cross-repressive interactions are required to stabilize photoreceptor and bipolar cell fates.© 2013, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved.

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This suggests that rods can directly convert into bipolar cells.

If true, progenitors that become postmitotic embryonically should adopt bipolar fate.

Because Kaede can be converted from green to red fluorescence at any developmental time point, it serves as a temporal landmark for cell birth.

When it is used in combination with subpopulation markers, the eventual fate of a cell can be correlated with its birthdate.

This protocol describes how the photoconvertible protein Kaede can be used to determine the birthdates of neurons in live zebrafish.

The methods used are birthdating analysis by photoconverted fluorescent protein tracing in vivo (BAPTI) and BAPTI combined with subpopulation markers (BAPTISM).

Since the two chemicals are analogous, some spots of the genetic code that call for Thymidine will instead receive Brd U.